ISG20 Antibody (1B2-3C9)
Novus Biologicals, part of Bio-Techne | Catalog # H00003669-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1B2-3C9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ISG20 (AAH07922.1, 1 a.a. ~ 181 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIRPEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDMSGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHSSVEDARATMELYQISQRIRARRGLPRLAVSD
Specificity
ISG20 - interferon stimulated gene 20kDa
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ISG20 Antibody (1B2-3C9)
![Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01]](https://resources.bio-techne.com/images/products/ISG20-Antibody-1B2-3C9-Western-Blot-H00003669-M01-img0009.jpg "Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01]")
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01]
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - BT-474, HCC1954 and SUM190 whole cell lysates were loaded with 40 ug/lane. 10% SDS-PAGE. ISG20 Antibody (H00003669-M01) was used for primary antibody: 1:1000, 4C, overnight. WB image submitted by a verified customer review.![Immunocytochemistry/ Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01]](https://resources.bio-techne.com/images/products/ISG20-Antibody-1B2-3C9-Immunocytochemistry-Immunofluorescence-H00003669-M01-img0006.jpg "Immunocytochemistry/ Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01]")
Immunocytochemistry/ Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01]
Immunocytochemistry/Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell . Antibody concentration 10 ug/ml.![Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01]](https://resources.bio-techne.com/images/products/ISG20-Antibody-1B2-3C9-Western-Blot-H00003669-M01-img0008.jpg "Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01]")
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01]
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: Non-transfected lysate.Applications for ISG20 Antibody (1B2-3C9)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using H00003669-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ISG20
Alternate Names
CD25, Estrogen-regulated transcript 45 protein, HEM45EC 3.1.13.1, interferon stimulated exonuclease gene 20kDa, interferon stimulated gene (20kD), interferon-stimulated gene 20 kDa protein, Promyelocytic leukemia nuclear body-associated protein ISG20
Entrez Gene IDs
3669 (Human)
Gene Symbol
ISG20
OMIM
604533 (Human)
UniProt
Additional ISG20 Products
Product Documents for ISG20 Antibody (1B2-3C9)
Product Specific Notices for ISG20 Antibody (1B2-3C9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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