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Islet-1 Antibody (1H9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47537

Novus Biologicals, part of Bio-Techne
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NBP1-47537
NBP1-47537-0.025ml

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H9

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified recombinant fragment of human Islet-1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Islet-1 Antibody (1H9) - BSA Free

Western Blot: Islet-1 Antibody (1H9)BSA Free [NBP1-47537]

Western Blot: Islet-1 Antibody (1H9)BSA Free [NBP1-47537]

Western Blot: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).
Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody (1H9) - BSA Free [NBP1-47537]

Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody (1H9) - BSA Free [NBP1-47537]

Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Islet-1 Antibody (1H9) - BSA Free [NBP1-47537]

Immunohistochemistry-Paraffin: Islet-1 Antibody (1H9) - BSA Free [NBP1-47537]

Immunohistochemistry-Paraffin: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using ISL1 mouse mAb with DAB staining.

Applications for Islet-1 Antibody (1H9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, ELISA.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Islet-1

ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Alternate Names

ISL1, Islet1

Entrez Gene IDs

3670 (Human)

Gene Symbol

ISL1

UniProt

Additional Islet-1 Products

Product Documents for Islet-1 Antibody (1H9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Islet-1 Antibody (1H9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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