ITK Antibody (5G6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37578

Novus Biologicals, part of Bio-Techne
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NBP2-37578
NBP2-37578-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G6

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all ITK Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ITK expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ITK Antibody (5G6) - BSA Free

Western Blot: ITK Antibody (5G6)BSA Free [NBP2-37578]

Western Blot: ITK Antibody (5G6)BSA Free [NBP2-37578]

Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mouse mAb against Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Immunocytochemistry/ Immunofluorescence: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Immunocytochemistry/Immunofluorescence: ITK Antibody (5G6) [NBP2-37578] - Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Flow Cytometry: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Flow Cytometry: ITK Antibody (5G6) [NBP2-37578] - Analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
View 2 more images

Applications for ITK Antibody (5G6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ITK

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Long Name

IL-2-inducible T-cell Kinase

Alternate Names

EMT, LYK, PSCTK2, Tcsk

Gene Symbol

ITK

Additional ITK Products

Product Documents for ITK Antibody (5G6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ITK Antibody (5G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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