ITK Antibody (8F10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15208

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-15208
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8F10

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ITK Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ITK. UniProt ID (Q08881).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ITK Antibody (8F10) - BSA Free

Immunohistochemistry: ITK Antibody (8F10) [NBP3-15208]

Immunohistochemistry: ITK Antibody (8F10) [NBP3-15208]

Immunohistochemistry: ITK Antibody (8F10) [NBP3-15208] - IHC image of ITK antibody diluted at 1:100 and staining in paraffin-embedded human lymph node tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for ITK Antibody (8F10) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ITK

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Long Name

IL-2-inducible T-cell Kinase

Alternate Names

EMT, LYK, PSCTK2, Tcsk

Gene Symbol

ITK

Additional ITK Products

Product Documents for ITK Antibody (8F10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ITK Antibody (8F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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