ITM2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36963

Novus Biologicals, part of Bio-Techne
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NBP1-36963
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ITM2B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HDKETYKLQRRETIK corresponding to internal region according to NP_068839.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ITM2B Antibody

Western Blot: ITM2B Antibody [NBP1-36963]

Western Blot: ITM2B Antibody [NBP1-36963]

Western Blot: ITM2B Antibody [NBP1-36963] - HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963]

Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963]

Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10u g/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963]

Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963]

Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963] - Human small intestine tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for ITM2B Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ITM2B

ITM2B is a type II membrane protein. It is expressed in brain and in other tissues. Defects in ITM2B seem to be associated with familial British dementia (FBD) and familial Danish dementia (FDD).

Long Name

Integral Membrane Protein 2B

Alternate Names

ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD

Entrez Gene IDs

9445 (Human); 16432 (Mouse); 290364 (Rat)

Gene Symbol

ITM2B

UniProt

NP_068839.1 (Human)

Additional ITM2B Products

Product Documents for ITM2B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ITM2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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