Jak2 Antibody (53B7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59451

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Insect

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Microarray, Western Blot

Cited:

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 53B7

Format

BSA Free

Concentration

1 mg/ml

View all Jak2 Antibodies »

Product Specifications

Immunogen

Recombinant human JAK2 (1014-1132aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Jak2 Antibody (53B7) - BSA Free

Western Blot: Jak2 Antibody (53B7)BSA Free [NBP2-59451]

Western Blot: Jak2 Antibody (53B7)BSA Free [NBP2-59451]

Western Blot: Jak2 Antibody (53B7) [NBP2-59451] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of JAK2 was detected. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Immunocytochemistry/ Immunofluorescence: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Immunocytochemistry/Immunofluorescence: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) and anti-human JAK2 antibody at 1:100 with goat anti-mouse IgG Alexa Fluor 488 conjugate (Green).
Flow Cytometry: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Flow Cytometry: Jak2 Antibody (53B7) - BSA Free [NBP2-59451]

Flow Cytometry: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cell line, antibody at 2 - 5 ug per 10^6cells (red line). Secondary antibody: goat anti-mouse IgG Alexa Fluor 488 conjugate. Isotype control antibody: mouse IgG (black line).
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Applications for Jak2 Antibody (53B7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
This Jak2 Antibody (53B7) is validated for microarray from a verified customer review.

Reviewed Applications

Read 3 reviews rated 5 using NBP2-59451 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Jak2

Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Gene Symbol

JAK2

Additional Jak2 Products

Product Documents for Jak2 Antibody (53B7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Jak2 Antibody (53B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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