JAM-A Antibody (OTI3H3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71876

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3H3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all JAM-A Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human F11R(NP_653087) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JAM-A Antibody (OTI3H3) - Azide and BSA Free

Western Blot: JAM-A Antibody (OTI3H3)Azide and BSA Free [NBP2-71876]

Western Blot: JAM-A Antibody (OTI3H3)Azide and BSA Free [NBP2-71876]

Western Blot: JAM-A Antibody (OTI3H3) - Azide and BSA Free [NBP2-71876] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JAM-A.
Immunocytochemistry/ Immunofluorescence: JAM-A Antibody (OTI3H3) - Azide and BSA Free [NBP2-71876]

Immunocytochemistry/ Immunofluorescence: JAM-A Antibody (OTI3H3) - Azide and BSA Free [NBP2-71876]

Immunocytochemistry/Immunofluorescence: JAM-A Antibody (OTI3H3) - Azide and BSA Free [NBP2-71876] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY JAM-A.
Immunohistochemistry: JAM-A Antibody (OTI3H3) - Azide and BSA Free [NBP2-71876]

Immunohistochemistry: JAM-A Antibody (OTI3H3) - Azide and BSA Free [NBP2-71876]

Immunohistochemistry: JAM-A Antibody (OTI3H3) - Azide and BSA Free [NBP2-71876] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for JAM-A Antibody (OTI3H3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JAM-A

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Long Name

Junctional Adhesion Molecule A

Alternate Names

CD321, F11R, JAMA, PAM-1

Gene Symbol

F11R

Additional JAM-A Products

Product Documents for JAM-A Antibody (OTI3H3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for JAM-A Antibody (OTI3H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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