Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all JAMP Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human JAMP protein (between amino acids 20-150) [UniProt Q9P055]

Localization

Endoplasmic Reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JAMP Antibody - BSA Free

Western Blot: JAMP AntibodyBSA Free [NBP2-36446]

Western Blot: JAMP AntibodyBSA Free [NBP2-36446]

Western Blot: JAMP Antibody [NBP2-36446] - Western blot analysis of JAMP in HEK293 and A431 cell lysates.
Immunocytochemistry/ Immunofluorescence: JAMP Antibody - BSA Free [NBP2-36446]

Immunocytochemistry/ Immunofluorescence: JAMP Antibody - BSA Free [NBP2-36446]

Immunocytochemistry/Immunofluorescence: JAMP Antibody [NBP2-36446] - JAMP antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Endoplasmic reticulum staining was observed.
Immunohistochemistry-Paraffin: JAMP Antibody - BSA Free [NBP2-36446]

Immunohistochemistry-Paraffin: JAMP Antibody - BSA Free [NBP2-36446]

Immunohistochemistry-Paraffin: JAMP Antibody [NBP2-36446] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal kidney using rabbit polyclonal JAMP antibody (NBP2-36446) at 10 ug/ml concentration. The cells of various tubules/ducts showed a weak to moderate cytoplasmic immunopositivity for JAMP protein.
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Applications for JAMP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JAMP

JAMP, better known as the JNK1/MAPK8 associated membrane protein, is a regulator of MAPK8 activity in response to various stress stimuli. This regulation is part of a broader response to ER stress which induces JAMP to facilitate the degradation of misfolded ER luminal proteins via recruitment of the ERAD (endoplasmic reticulum-associated degradation system). Aside from interactions with MAPK8, JAMP also interacts with RNF5, as well as many regulatory proteins in the ERAD such as AMFR/GP78, CANX, PSMC1/PSMC2 and PSMC5-8. JAMP is expressed in many tissues, including brain, spleen, thymus, liver, kidney and testis. It also exhibits elevated expression in medulloblastomas. JAMP undergoes a post-translational modification after ubiquitination by RNF5 in a UBE2N-dependent manner, which ultimately decreases association with the proteasome and ERAD. Research has confirmed that JAMP regulates JNK-1 activity (PMID: 16166642) as well as optimizes ERAD to protect cells from unfolded proteins (PMID 18784250) which may have various implications in disease models.

Alternate Names

C14orf100, chromosome 14 open reading frame 100, HSPC213, HSPC327, JAMPCDA06, JNK1/MAPK8-associated membrane protein, JNK1-associated membrane protein, JNK-associated membrane protein, Jun N-terminal kinase 1-associated membrane protein, Medulloblastoma antigen MU-MB-50.4

Entrez Gene IDs

51528 (Human)

Gene Symbol

JKAMP

UniProt

Q9P055 (Human)

Additional JAMP Products

Product Documents for JAMP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for JAMP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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