JMJD2D Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03357
![Western Blot: JMJD2D AntibodyBSA Free [NBP1-03357]](https://resources.bio-techne.com/images/products/JMJD2D-Antibody-Western-Blot-NBP1-03357-img0002.jpg "Western Blot: JMJD2D AntibodyBSA Free [NBP1-03357]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Primate (92%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), ICC/IF (Negative), Western Blot
Cited:
Chemotaxis, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to a C-terminal portion of the human JMJD2D protein (within residues 460-520). [Swiss-Prot# Q6B0I6]
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: monkey (92%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for JMJD2D Antibody - BSA Free
Western Blot: JMJD2D AntibodyBSA Free [NBP1-03357]
Western Blot: JMJD2D Antibody [NBP1-03357] - Western Blot Image of anti-JMJD2D. Whole cell protein from PC3 (lane 1), SHSY5Y (lane 2), and HeLa (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-JMJD2D in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Applications for JMJD2D Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation
reported in scientific literature
Western Blot
0.2 ug/ml
Application Notes
This JMJD2D antibody is useful for Western blot, where a band is seen at ~55 kDa. This antibody is not useful for ICC/IF. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 reviews rated 5 using NBP1-03357 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: JMJD2D
Long Name
Lysine-specific demethylase 4D
Alternate Names
JHDM3D, KDM4D
Entrez Gene IDs
55693 (Human)
Gene Symbol
KDM4D
UniProt
Additional JMJD2D Products
Product Documents for JMJD2D Antibody - BSA Free
Product Specific Notices for JMJD2D Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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