JNK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30905

Novus Biologicals, part of Bio-Techne
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NBP1-30905
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Insect

Predicted:

Chicken (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all JNK2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human JNK2. The exact sequence is proprietary.

Reactivity Notes

Reacts with Plutella xylostella.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JNK2 Antibody

Western Blot: JNK2 Antibody [NBP1-30905]

Western Blot: JNK2 Antibody [NBP1-30905]

Western Blot: JNK2 Antibody [NBP1-30905] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: JNK2 Antibody [NBP1-30905]

Immunocytochemistry/ Immunofluorescence: JNK2 Antibody [NBP1-30905]

Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP1-30905] - Analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: JNK2 Antibody [NBP1-30905]

Immunohistochemistry-Paraffin: JNK2 Antibody [NBP1-30905]

Immunohistochemistry-Paraffin: JNK2 Antibody [NBP1-30905] - Paraffin-embedded N87 xenograft, using antibody at 1:100 dilution.
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Applications for JNK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JNK2

JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Long Name

C-Jun N-terminal Kinase 2

Alternate Names

MAPK9, SAPK1 alpha

Gene Symbol

MAPK9

Additional JNK2 Products

Product Documents for JNK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for JNK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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