JNK3 Antibody (29E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26149

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-26149-100ul
NBP3-26149-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 29E10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JNK3 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human JNK3 [UniProt P53779]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JNK3 Antibody (29E10)

JNK3 Antibody (29E10)

Immunohistochemistry: JNK3 Antibody (29E10) [NBP3-26149] -

Immunohistochemistry: JNK3 Antibody (29E10) [NBP3-26149] - Image of JNK3 Antibody (29E10) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
JNK3 Antibody (29E10)

Western Blot: JNK3 Antibody (29E10) [NBP3-26149] -

Western Blot: JNK3 Antibody (29E10) [NBP3-26149] - Positive Western Blot detected in: PC-3 whole cell lysate, Mouse brain tissue.
All lanes: JNK3 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 53, 49, 32kDa
Observed band size: 55 kDa
JNK3 Antibody (29E10)

Immunocytochemistry/Immunofluorescence: JNK3 Antibody (29E10) [NBP3-26149] -

Immunocytochemistry/Immunofluorescence: JNK3 Antibody (29E10) [NBP3-26149] - Staining of PC-3 cell with JNK3 Antibody (29E10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 553-conjugated Goat Anti-Rabbit IgG (H+L).
View 1 more images

Applications for JNK3 Antibody (29E10)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: JNK3

MAPK10 (JNK3) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuron-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 (CDK5) can phosphorylate and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

C-Jun N-terminal Kinase 3

Alternate Names

MAPK10, SAPK1 beta

Gene Symbol

MAPK10

Additional JNK3 Products

Product Documents for JNK3 Antibody (29E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for JNK3 Antibody (29E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.