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JunB/AP-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51950

Novus Biologicals, part of Bio-Techne
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NBP1-51950

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-TLKAENAGLSSTAG corresponding to internal region according to NP_002220.1.

Epitope

C-TLKAENAGLSSTAG

Specificity

This antibody may also cross-react with human, mouse, rat, cow, dog JUN (GeneID: 3725, 16476, 24516, 280831, 609429).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for JunB/AP-1 Antibody

Western Blot: JunB/AP-1 Antibody [NBP1-51950]

Western Blot: JunB/AP-1 Antibody [NBP1-51950]

Western Blot: JunB/AP-1 Antibody [NBP1-51950] - Analysis of JunB in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NBP1-51950 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for JunB/AP-1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human breast cancer lysates (calculated MW of 35.9 kDa band according to NP_002220.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JunB

The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge.

Long Name

JunB Proto-oncogene

Alternate Names

activator protein 1, AP-1, jun B proto-oncogene, transcription factor jun-B

Entrez Gene IDs

3726 (Human); 16477 (Mouse); 24517 (Rat)

Gene Symbol

JUNB

Additional JunB Products

Product Documents for JunB/AP-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for JunB/AP-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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