JunD Antibody (2D5A1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16602

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-16602-100ul
NBP3-16602-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2D5A1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JunD Antibodies »

Product Summary for JunD Antibody (2D5A1)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human JunD (P17535). METPFYGDEALSGLGGGASGSGGSFASPGRLFPGAPPTAAAGSMMKKDALTLSLSEQVAAALKPAAAPPPTPLRADGAPSAAPPDGLLASPDLGLLKLASPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JunD Antibody (2D5A1)

Western Blot: JunD Antibody (2D5A1) [NBP3-16602]

Western Blot: JunD Antibody (2D5A1) [NBP3-16602]

Western Blot: JunD Antibody (2D5A1) [NBP3-16602] - Western blot analysis of extracts of various cell lines, using JunD antibody (NBP3-16602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: JunD Antibody (2D5A1) [NBP3-16602]

Immunohistochemistry-Paraffin: JunD Antibody (2D5A1) [NBP3-16602]

Immunohistochemistry-Paraffin: JunD Antibody (2D5A1) [NBP3-16602] - Immunohistochemistry of paraffin-embedded mouse spleen using JunD Rabbit mAb (NBP3-16602) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: JunD Antibody (2D5A1) [NBP3-16602]

Immunohistochemistry-Paraffin: JunD Antibody (2D5A1) [NBP3-16602]

Immunohistochemistry-Paraffin: JunD Antibody (2D5A1) [NBP3-16602] - Immunohistochemistry of paraffin-embedded rat lung using JunD Rabbit mAb (NBP3-16602) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
View 2 more images

Applications for JunD Antibody (2D5A1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JunD

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Gene Symbol

JUND

Additional JunD Products

Product Documents for JunD Antibody (2D5A1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for JunD Antibody (2D5A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.