JunD Antibody (5L7Y3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16600

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16600-100ul
NBP3-16600-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5L7Y3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JunD Antibodies »

Product Summary for JunD Antibody (5L7Y3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human JunD (P17535). SPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALEDLHKQNQLGAGAAAAAAAAAAGGPSGTATGSAPPGELAPAAAAPEAPVYANLSSY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JunD Antibody (5L7Y3)

Western Blot: JunD Antibody (5L7Y3) [NBP3-16600]

Western Blot: JunD Antibody (5L7Y3) [NBP3-16600]

Western Blot: JunD Antibody (5L7Y3) [NBP3-16600] - Western blot analysis of extracts of various cell lines, using JunD Rabbit mAb (NBP3-16600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Chromatin Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using JunD antibody (NBP3-16600) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600]

Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600]

Immunoprecipitation: JunD Antibody (5L7Y3) [NBP3-16600] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug JunD antibody (NBP3-16600). Western blot was performed from the immunoprecipitate using JunD antibody (NBP3-16600) at a dilition of 1:1000.

Applications for JunD Antibody (5L7Y3)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JunD

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Gene Symbol

JUND

Additional JunD Products

Product Documents for JunD Antibody (5L7Y3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JunD Antibody (5L7Y3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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