Kaiso Antibody (10O4V2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16272

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16272-100ul
NBP3-16272-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10O4V2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Kaiso Antibodies »

Product Summary for Kaiso Antibody (10O4V2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 573-672 of human Kaiso (Q86T24). HSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kaiso Antibody (10O4V2)

Western Blot: Kaiso Antibody (10O4V2) [NBP3-16272]

Western Blot: Kaiso Antibody (10O4V2) [NBP3-16272]

Western Blot: Kaiso Antibody (10O4V2) [NBP3-16272] - Western blot analysis of extracts of various cell lines, using Kaiso Rabbit mAb (NBP3-16272) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Kaiso Antibody (10O4V2) [NBP3-16272]

Immunocytochemistry/ Immunofluorescence: Kaiso Antibody (10O4V2) [NBP3-16272]

Immunocytochemistry/Immunofluorescence: Kaiso Antibody (10O4V2) [NBP3-16272] - Immunofluorescence analysis of HeLa cells using Kaiso Rabbit mAb (NBP3-16272) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: Kaiso Antibody (10O4V2) [NBP3-16272]

Immunoprecipitation: Kaiso Antibody (10O4V2) [NBP3-16272]

Immunoprecipitation: Kaiso Antibody (10O4V2) [NBP3-16272] - Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Kaiso antibody (NBP3-16272). Western blot was performed from the immunoprecipitate using Kaiso antibody (NBP3-16272) at a dilition of 1:1000.

Applications for Kaiso Antibody (10O4V2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kaiso

KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA (Filion et al., 2006 (PubMed 16354688)).(supplied by OMIM)

Alternate Names

KAISOkaiso transcription factor, transcriptional regulator Kaiso, WUGSC:H_DJ525N14.1, zinc finger and BTB domain containing 33, Zinc finger and BTB domain-containing protein 33, ZNF348kaiso, ZNF-kaiso

Gene Symbol

ZBTB33

Additional Kaiso Products

Product Documents for Kaiso Antibody (10O4V2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Kaiso Antibody (10O4V2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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