Kaiso Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35285
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation, ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human Kaiso (NP_006768.1).
Sequence:
PCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGKSFINYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY
Sequence:
PCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGKSFINYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Kaiso Antibody
Western Blot: Kaiso Antibody [NBP3-35285] -
Western Blot: Kaiso Antibody [NBP3-35285] - Western blot analysis of various lysates using Kaiso Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Chromatin Immunoprecipitation: Kaiso Antibody [NBP3-35285] -
Chromatin Immunoprecipitation: Kaiso Antibody [NBP3-35285] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Applications for Kaiso Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation
5ug antibody for 10ug-15ug of Chromatin
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Kaiso
Alternate Names
KAISOkaiso transcription factor, transcriptional regulator Kaiso, WUGSC:H_DJ525N14.1, zinc finger and BTB domain containing 33, Zinc finger and BTB domain-containing protein 33, ZNF348kaiso, ZNF-kaiso
Gene Symbol
ZBTB33
Additional Kaiso Products
Product Documents for Kaiso Antibody
Product Specific Notices for Kaiso Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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