Kallikrein 3/PSA Antibody (5A11E9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51541

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5A11E9

Format

BSA Free

Concentration

1.0 mg/ml

View all Kallikrein 3/PSA Antibodies »

Product Summary for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free

Immunogen

Purified recombinant fragment of Prostate Specific Antigen (amino acids 26-251) expressed in E. coli. [UniProt# P07288]

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free

Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]

Western Blot: Kallikrein 3/PSA Antibody (5A11E9)BSA Free [NBP1-51541]

Western Blot: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis against truncated Prostate Specific Antigen recombinant protein.
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]

Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]

Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using Prostate Specific Antigen mouse mAb with DAB staining.
Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]

Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) - BSA Free [NBP1-51541]

Flow Cytometry: Kallikrein 3/PSA Antibody (5A11E9) [NBP1-51541] - Flow cytometric analysis of PC-3 cells using Prostate Specific Antigen mouse mAb (right) and negative control (left).
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Applications for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This Prostate Specific Antigen (5A11E9) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kallikrein 3/PSA

Prostate Specific Antigen (PSA, Kallikrein-3, KLK3) is a serine protease that is usually secreted from the prostate gland and presented in seminal plasma. Prostate Specific Antigen functions in the liquefaction of seminal coagulum, through hydrolysis of the high molecular mass seminal vesicle protein. Its substrates includes semenogelin I & II, fibronectin, insulin-like growth factor binding protein 3 (IGBP3), and laminin. Highly expressed by normal and malignant prostate cancer cells, Prostate Specific Antigen has been implicated in the growth, invasion, and metastasis of prostate cancer cells. Therefore, Prostate Specific Antigen is widely used as a diagnostic marker for prostate cancer.

Alternate Names

KLK3, PSA

Entrez Gene IDs

354 (Human)

Gene Symbol

KLK3

UniProt

P07288

Additional Kallikrein 3/PSA Products

Product Documents for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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