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Kallikrein 3/PSA Antibody (RM323)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77416

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-77416

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM323

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Native Kallikrein 3/PSA protein purified from human seminal fluid

Specificity

This antibody reacts to native human Kallikrein 3/PSA, including both free (unbound) form and in a complex with ACT (alpha 1-antichymotrypsin). It may react to reduced Kallikrein 3/PSA at a higher concentration.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kallikrein 3/PSA Antibody (RM323)

Western Blot: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]

Western Blot: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]

Western Blot: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416] - Western Blot of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non-reduced (-) or reduced (+) conditions, using NBP2-77416.
Immunohistochemistry: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]

Immunohistochemistry: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]

Immunohistochemistry: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416] - Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer tissue section using NBP2-77416.
ELISA: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]

ELISA: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416]

ELISA: Kallikrein 3/PSA Antibody (RM323) [NBP2-77416] - A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of NBP2-77416 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for Kallikrein 3/PSA Antibody (RM323)

Application
Recommended Usage

ELISA

1:100 - 1:5000

Immunohistochemistry

1:500 - 1:1000

Western Blot

1:1000 - 1:2500 (non-reducing conditions)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kallikrein 3/PSA

PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liverderived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Alternate Names

KLK3, PSA

Gene Symbol

KLK3

Additional Kallikrein 3/PSA Products

Product Documents for Kallikrein 3/PSA Antibody (RM323)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Kallikrein 3/PSA Antibody (RM323)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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