KAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1161

Novus Biologicals, part of Bio-Techne
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NB100-1161
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all KAP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SSQELSGGPGDGP corresponding to C-Terminus according to NP_005753.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for KAP1 Antibody

Immunocytochemistry/ Immunofluorescence: KAP1 Antibody [NB100-1161]

Immunocytochemistry/ Immunofluorescence: KAP1 Antibody [NB100-1161]

Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: KAP1 Antibody [NB100-1161]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB100-1161]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB100-1161] - (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: KAP1 Antibody [NB100-1161]

Flow Cytometry: KAP1 Antibody [NB100-1161]

Flow Cytometry: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for KAP1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2-4 ug/ml

Immunohistochemistry-Paraffin

2-4 ug/ml

Peptide ELISA

Detection limit 1:2000
Application Notes
Immunofluorescence: Successful usage on mouse embryo at the late zygote stage, 5ug/ml. Strong expression of the protein seen in the nucleus of HeLa and U2OS cells.
ChIP successful usage for K562 cells is reported in scientific literature (PMID: 21170338). WB: Approx. 120 kDa band observed in HepG2 lysates (calculated MW of 88.5 kDa band according to NP_005753.1). The higher observed MW is explained by sumoylated protein.
IHC-P: Human breast shows nuclear staining in epithelial duct cells. IHC successful usage on human gastric cancer surgical specimens is reported in scientific literature (PMID: 19898899).
Flow Cytometry: Flow cytometric analysis of HeLa cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KAP1

The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1). KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Entrez Gene IDs

10155 (Human); 21849 (Mouse)

Gene Symbol

TRIM28

UniProt

NP_005753. (Human)

Additional KAP1 Products

Product Documents for KAP1 Antibody

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Product Specific Notices for KAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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