KAP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-158
![Western Blot: KAP1 Antibody [NB500-158]](https://resources.bio-techne.com/images/products/KAP1-Antibody-Western-Blot-NB500-158-img0024.jpg "Western Blot: KAP1 Antibody [NB500-158]")
![Western Blot: KAP1 Antibody [NB500-158]](https://resources.bio-techne.com/images/products/KAP1-Antibody-Western-Blot-NB500-158-img0025.jpg "Western Blot: KAP1 Antibody [NB500-158]")
![Immunoprecipitation: KAP1 Antibody [NB500-158]](https://resources.bio-techne.com/images/products/KAP1-Antibody-Immunoprecipitation-NB500-158-img0026.jpg "Immunoprecipitation: KAP1 Antibody [NB500-158]")
![Western Blot: KAP1 Antibody [NB500-158]](https://resources.bio-techne.com/images/products/KAP1-Antibody-Western-Blot-NB500-158-img0028.jpg "Western Blot: KAP1 Antibody [NB500-158]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-2350, NB100-2241
Scientific Data Images for KAP1 Antibody - BSA Free
Western Blot: KAP1 Antibody [NB500-158]
Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158]](https://resources.bio-techne.com/images/products/KAP1-Antibody-Immunohistochemistry-NB500-158-img0022.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158]")
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158]
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158] - FFPE sections of human breast carcinoma. KAP1 antibody Lot3 (left) and Lot2 (right) used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.Applications for KAP1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500- 1:2500
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
2-10 ug/mg of lysate
Western Blot
1:2000-1:10000
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. KAP1 antibody validated for WB from a verified customer review.
Reviewed Applications
Read 1 review rated 5 using NB500-158 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: KAP1
Long Name
KRAB-associated Protein 1
Alternate Names
KRIP-1, RNF96, TF1B, TIF1B, TRIM28
Entrez Gene IDs
10155 (Human)
Gene Symbol
TRIM28
UniProt
Additional KAP1 Products
Product Documents for KAP1 Antibody - BSA Free
Product Specific Notices for KAP1 Antibody - BSA Free
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-2350, NB100-2241
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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