KAP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-159
![Western Blot: KAP1 Antibody [NB500-159] Western Blot: KAP1 Antibody [NB500-159]](https://resources.bio-techne.com/images/products/KAP1-Antibody-Western-Blot-NB500-159-img0010.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KAP1 Antibody - BSA Free
Western Blot: KAP1 Antibody [NB500-159]
Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of mouse plasmacytoma Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).Applications for KAP1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 to 1:5000
Immunohistochemistry-Paraffin
1:1000 to 1:5000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~106 kDa, representing KAP-1. The investigator should determine the optimal working dilution for a specific application. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: KAP1
Long Name
KRAB-associated Protein 1
Alternate Names
KRIP-1, RNF96, TF1B, TIF1B, TRIM28
Entrez Gene IDs
10155 (Human)
Gene Symbol
TRIM28
UniProt
Additional KAP1 Products
Product Documents for KAP1 Antibody - BSA Free
Product Specific Notices for KAP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...