Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all KAP1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAP1 Antibody

Western Blot: KAP1 Antibody [NB500-159]

Western Blot: KAP1 Antibody [NB500-159]

Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of mouse plasmacytoma Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
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Applications for KAP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 to 1:5000

Immunohistochemistry-Paraffin

1:1000 to 1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~106 kDa, representing KAP-1. The investigator should determine the optimal working dilution for a specific application. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KAP1

The nuclear corepressor, KAP-1, is involved in silencing heterochromatin. It has also been suggested to play a role in signal transduction.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Entrez Gene IDs

10155 (Human)

Gene Symbol

TRIM28

UniProt

NP_005753.1 (Human)

Additional KAP1 Products

Product Documents for KAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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