KAP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32072
![Western Blot: KAP1 Antibody [NBP2-32072] Western Blot: KAP1 Antibody [NBP2-32072]](https://resources.bio-techne.com/images/products/KAP1-Antibody-Western-Blot-NBP2-32072-img0001.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 550 to 600 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_035718.2 (GeneID 21849).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KAP1 Antibody - BSA Free
Western Blot: KAP1 Antibody [NBP2-32072]
Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Immunoprecipitation: KAP1 Antibody [NBP2-32072]
Immunoprecipitation: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32072. For blotting immunoprecipitated KAP-1, NBP2-32072 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for KAP1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KAP1
Long Name
KRAB-associated Protein 1
Alternate Names
KRIP-1, RNF96, TF1B, TIF1B, TRIM28
Gene Symbol
TRIM28
UniProt
Additional KAP1 Products
Product Documents for KAP1 Antibody - BSA Free
Product Specific Notices for KAP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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