KAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-39014

Novus Biologicals, part of Bio-Techne
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NBP2-39014
NBP2-39014-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KAP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAP1 Antibody

Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014]

Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014]

Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014] - Staining in human ovary and liver tissues using anti-TRIM28 antibody. Corresponding TRIM28 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: KAP1 Antibody [NBP2-39014]

Immunocytochemistry/ Immunofluorescence: KAP1 Antibody [NBP2-39014]

Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NBP2-39014] - Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014]

Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014]

Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014] - Staining of human liver shows low expression as expected.
View 1 more images

Applications for KAP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KAP1

KAP1 is encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Gene Symbol

TRIM28

UniProt

Q13263 (Human)

Additional KAP1 Products

Product Documents for KAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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