Kappa Light Chain Antibody (8O7Q9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15596
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8O7Q9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Kappa Light Chain Antibody (8O7Q9)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human Kappa light chain (P01601). MDMRVLAQLLGLLLLCFPGARCDIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPEKAPKSLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Kappa Light Chain Antibody (8O7Q9)
![Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]](https://resources.bio-techne.com/images/products/Kappa-Light-Chain-Antibody-8O7Q9-Western-Blot-NBP3-15596-img0002.jpg "Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]")
Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]
Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596] - Western blot analysis of extracts of Human plasma cells, using Kappa Light Chain antibody (NBP3-15596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]](https://resources.bio-techne.com/images/products/Kappa-Light-Chain-Antibody-8O7Q9-Immunohistochemistry-Paraffin-NBP3-15596-img0001.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]")
Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]
Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596] - Immunohistochemistry of paraffin-embedded human tonsil using Kappa Light Chain Rabbit mAb (NBP3-15596) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for Kappa Light Chain Antibody (8O7Q9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Kappa Light Chain Antibody (8O7Q9)
Product Specific Notices for Kappa Light Chain Antibody (8O7Q9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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