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Kappa Light Chain Antibody (A8B5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45067

Novus Biologicals, part of Bio-Techne
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NBP1-45067
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A8B5

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Kappa Light Chain Antibody (A8B5) - BSA Free

Immunogen

The antibody A8B5 reacts withIGKC (22.5 kDa) of immunoglobulins.

Specificity

The mouse monoclonal antibody 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kappa Light Chain Antibody (A8B5) - BSA Free

Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]

Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]

Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - Surface staining of immunoglobulin kappa light chains in human peripheral blood with anti-kappa light chains (A8B5) Pacific BlueTM.
Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]

Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]

Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.
Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]

Flow Cytometry: Kappa Light Chain Antibody (A8B5) - BSA Free [NBP1-45067]

Flow Cytometry: Kappa Light Chain Antibody (A8B5) [NBP1-45067] - Surface staining of immunoglobulin kappa light chains in human peripheral blood with anti-kappa light chains (A8B5) PE.

Applications for Kappa Light Chain Antibody (A8B5) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Kappa Light Chain Antibody (A8B5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Kappa Light Chain Antibody (A8B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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