Kappa Light Chain Antibody (MEM-09) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-331
![Western Blot: Kappa Light Chain Antibody (MEM-09)BSA Free [NB500-331]](https://resources.bio-techne.com/images/products/Kappa-Light-Chain-Antibody-MEM-09-Western-Blot-NB500-331-img0001.jpg "Western Blot: Kappa Light Chain Antibody (MEM-09)BSA Free [NB500-331]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-09
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for Kappa Light Chain Antibody (MEM-09) - BSA Free
Immunogen
Crude thymus membrane fraction.
Specificity
The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Kappa Light Chain Antibody (MEM-09) - BSA Free
Western Blot: Kappa Light Chain Antibody (MEM-09)BSA Free [NB500-331]
Western Blot: Kappa Light Chain Antibody (MEM-09) [NB500-331] - Analysis (reducing conditions) of human blood sera using anti-human Immunoglobulin kappa-chain (MEM-09). Lane 1-3: Human blood serum of different healthy donorsApplications for Kappa Light Chain Antibody (MEM-09) - BSA Free
Application
Recommended Usage
ELISA
1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:50
Western Blot
1:1000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Entrez Gene IDs
3514 (Human)
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Kappa Light Chain Antibody (MEM-09) - BSA Free
Product Specific Notices for Kappa Light Chain Antibody (MEM-09) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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