Kappa Light Chain Antibody (RM103)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62003
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM103
Concentration
1 mg/ml
Product Summary for Kappa Light Chain Antibody (RM103)
Immunogen
Mouse IgG
Specificity
This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Kappa Light Chain Antibody (RM103)
Western Blot: Kappa Light Chain Antibody (RM103) [Unconjugated] [NBP2-62003] - Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa and IgG1 lambda, using NBP2-62003. This antibody reacts to nonreduced IgG1k (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
ELISA: Kappa Light Chain Antibody (RM103) [Unconjugated] [NBP2-62003] - ELISA of mouse immunoglobulins shows NBP2-62003 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG (kappa+lambda), rat IgG (kappa+lambda), or goat IgG (kappa+lambda). The plate was coated with different immunoglobulins.NBP2-62003 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: Kappa Light Chain Antibody (RM103) [Unconjugated] [NBP2-62003] - A titer ELISA of mouse IgG1 kappa. The plate was coated with different amounts of mouse IgG1 kappa. A serial dilution of NBP2-62003 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for Kappa Light Chain Antibody (RM103)
Application
Recommended Usage
ELISA
0.005ug/ml-0.2ug/ml
Western Blot
0.1ug/ml-0.5ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Kappa Light Chain Antibody (RM103)
Product Specific Notices for Kappa Light Chain Antibody (RM103)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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