Kappa Light Chain Antibody (RM103) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62003B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM103
Concentration
1 mg/ml
Product Summary for Kappa Light Chain Antibody (RM103) [Biotin]
Immunogen
Mouse IgG
Specificity
This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Kappa Light Chain Antibody (RM103) [Biotin]
Western Blot: Kappa Light Chain Antibody (RM103) [Biotin] [NBP2-62003B] - Western blot of nonreduced(-) and reduced(+) mouse IgG1(kappa) and IgG1(lambda) (20 ng/lane), using 0.2 ug/mL of clone RM103. This antibody reacts to nonreduced IgG1(kappa) (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
ELISA: Kappa Light Chain Antibody (RM103) [Biotin] [NBP2-62003B] - A titer ELISA of mouse IgG1 kappa. The plate was coated with different amounts of mouse IgG1 kappa. A serial dilution of NBP2-62003B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: Kappa Light Chain Antibody (RM103) [Biotin] [NBP2-62003B] - ELISA of mouse immunoglobulins shows RM103 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for Kappa Light Chain Antibody (RM103) [Biotin]
Application
Recommended Usage
ELISA
0.005ug/ml-0.2ug/ml
Western Blot
0.1ug/ml - 0.5ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Kappa Light Chain Antibody (RM103) [Biotin]
Product Specific Notices for Kappa Light Chain Antibody (RM103) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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