Karyopherin (importin) beta 3 Antibody (1C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00003843-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Karyopherin (importin) beta 3 Antibodies »

Product Specifications

Immunogen

RANBP5 (AAH01497, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAAAEQQQFYLLLGNLLSPDNVVRKQAEETYENIPGQSKITFLLQAIRNTTAAEEARQMAAVLLRRLLSSAFDEVYPALPSDVQTAIKSELLMIIQME

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic and Nuclear. Note=Found particularly in the nuclear rim and nucleolus.

Specificity

RANBP5 - RAN binding protein 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Karyopherin (importin) beta 3 Antibody (1C4)

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01]

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01]

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Analysis of IPO5 expression in transfected 293T cell line by RANBP5 monoclonal antibody (M01), clone 1C4.Lane 1: IPO5 transfected lysate (Predicted MW: 125.6 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01]

Immunohistochemistry-Paraffin: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01]

Immunohistochemistry-Paraffin: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - Analysis of monoclonal antibody to RANBP5 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01]

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01]

Western Blot: Karyopherin (importin) beta 3 Antibody (1C4) [H00003843-M01] - RANBP5 monoclonal antibody (M01), clone 1C4 Analysis of RANBP5 expression in HeLa.
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Applications for Karyopherin (importin) beta 3 Antibody (1C4)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Karyopherin (importin) beta 3

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Alternate Names

DKFZp686O1576, FLJ43041, IMB3, Imp5, importin 5, importin beta-3 subunit, Importin subunit beta-3, importin-5, karyopherin (importin) beta 3, Karyopherin beta-3, KPNB3, MGC2068, RAN binding protein 5, Ran_GTP binding protein 5, Ran-binding protein 5, RanBP5

Entrez Gene IDs

3843 (Human)

Gene Symbol

IPO5

OMIM

602008 (Human)

UniProt

AAH01497 (Human)

Additional Karyopherin (importin) beta 3 Products

Product Documents for Karyopherin (importin) beta 3 Antibody (1C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Karyopherin (importin) beta 3 Antibody (1C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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