Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all KCNJ10 Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the mid-protein of Human KCNJ10

Localization

Membrane|Multi-Pass Membrane Protein|Basolateral Cell Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KCNJ10 Antibody - BSA Free

Western Blot: KCNJ10 Antibody [NBP3-18248]

Western Blot: KCNJ10 Antibody [NBP3-18248]

Western Blot: KCNJ10 Antibody [NBP3-18248] - Western blot analysis of Rat Liver cell lysates showing detection of ~42.5 kDa KCNJ10 protein using Rabbit Anti-KCNJ10 Polyclonal Antibody (NBP3-18248). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KCNJ10 Polyclonal Antibody (NBP3-18248) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42.5 kDa.
Immunocytochemistry/ Immunofluorescence: KCNJ10 Antibody [NBP3-18248]

Immunocytochemistry/ Immunofluorescence: KCNJ10 Antibody [NBP3-18248]

Immunocytochemistry/Immunofluorescence: KCNJ10 Antibody [NBP3-18248] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KCNJ10 Polyclonal Antibody (NBP3-18248). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-KCNJ10 Polyclonal Antibody (NBP3-18248) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) KCNJ10 Antibody. (D) Composite.
Immunohistochemistry: KCNJ10 Antibody [NBP3-18248]

Immunohistochemistry: KCNJ10 Antibody [NBP3-18248]

Immunohistochemistry: KCNJ10 Antibody [NBP3-18248] - Immunohistochemistry analysis using Rabbit Anti-KCNJ10 Polyclonal Antibody (NBP3-18248). Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-KCNJ10 Polyclonal Antibody (NBP3-18248) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Applications for KCNJ10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS, 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KCNJ10

KCNJ10 encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes.

Long Name

ATP-sensitive inward rectifier potassium channel 10

Alternate Names

ATP-dependent inwardly rectifying potassium channel Kir4.1, ATP-sensitive inward rectifier potassium channel 10, BIRK-10, glial ATP-dependent inwardly rectifying potassium channel KIR4.1, Inward rectifier K(+) channel Kir1.2, inward rectifier K+ channel KIR1.2, KCNJ13-PEN, KIR1.2, Kir4.1, Potassium channel, inwardly rectifying subfamily J member 10, potassium inwardly-rectifying channel, subfamily J, member 10, SESAME

Gene Symbol

KCNJ10

Additional KCNJ10 Products

Product Documents for KCNJ10 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KCNJ10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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