KDM6A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80628
![Western Blot: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Western-Blot-NBP1-80628-img0021.jpg "Western Blot: KDM6A Antibody [NBP1-80628]")
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0020.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (94%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHT
Reactivity Notes
Mouse reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KDM6A Antibody
![Western Blot: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Western-Blot-NBP1-80628-img0022.jpg "Western Blot: KDM6A Antibody [NBP1-80628]")
Western Blot: KDM6A Antibody [NBP1-80628]
Western Blot: KDM6A Antibody [NBP1-80628] - HMLE cells were transduced with either an empty vector (HMLE-Vector), or genes encoding the EMT-inducing transcription factors Snail (HMLE-Snail) or Twist (HMLE-Twist) [4]. Whole-cell lysates were analyzed by immunoblotting for KDM6A. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29029452/) licensed under a CC-BY license.![Western Blot: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Western-Blot-NBP1-80628-img0006.jpg "Western Blot: KDM6A Antibody [NBP1-80628]")
Western Blot: KDM6A Antibody [NBP1-80628]
Western Blot: KDM6A Antibody [NBP1-80628] - Analysis in human cell line HEK 293.![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0012.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]")
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0013.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]")
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0016.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]")
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0017.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]")
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0018.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]")
![Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]](https://resources.bio-techne.com/images/products/KDM6A-Antibody-Immunohistochemistry-Paraffin-NBP1-80628-img0019.jpg "Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]")
Applications for KDM6A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID: 32071397).
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using NBP1-80628 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lysine (K)-specific Demethylase 6A/KDM6A/UTX
Alternate Names
KABUK2, KDM6A, Lysine (K)specific Demethylase 6A, UTX
Entrez Gene IDs
7403 (Human)
Gene Symbol
KDM6A
Additional Lysine (K)-specific Demethylase 6A/KDM6A/UTX Products
Product Documents for KDM6A Antibody
Product Specific Notices for KDM6A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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