Keap1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78793

Novus Biologicals, part of Bio-Techne
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NBP1-78793
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Keap1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EVTPSQHGNRTFS corresponding to internal region (near N-Terminus) according to NP_036421.2.

Epitope

C-EVTPSQHGNRTFS

Specificity

Reported variants represent identical protein: NP_036421.2, NP_987096.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Keap1 Antibody

Immunocytochemistry/ Immunofluorescence: Keap1 Antibody [NBP1-78793]

Immunocytochemistry/ Immunofluorescence: Keap1 Antibody [NBP1-78793]

Immunocytochemistry/Immunofluorescence: Keap1 Antibody [NBP1-78793] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Keap1 Antibody [NBP1-78793]

Immunohistochemistry-Paraffin: Keap1 Antibody [NBP1-78793]

Immunohistochemistry-Paraffin: Keap1 Antibody [NBP1-78793] - Staining of paraffin embedded Human Cerebellum with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Keap1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Immunohistochemistry

3 - 5 ug/mL

Immunohistochemistry-Paraffin

3 - 5 ug/mL

Peptide ELISA

Detection limit 1:32000
Application Notes
IHC-P: Human cerebellum shows textured cytoplasm staining in Purkinje cell and processes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Keap1

The KEAP1 gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associatedprotein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins inthe cytoplasm is followed

Long Name

Kelch-like ECH-associated Protein 1

Alternate Names

INrf2

Entrez Gene IDs

9817 (Human); 50868 (Mouse); 117519 (Rat)

Gene Symbol

KEAP1

UniProt

NP_036421.2 (Human)

Additional Keap1 Products

Product Documents for Keap1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Keap1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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