Keap1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17036
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (98%), Mouse (96%), Porcine (99%), Primate (100%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Keap1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human KEAP1. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%).
Localization
Cytoplasm; Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Keap1 Antibody
![Immunocytochemistry/ Immunofluorescence: Keap1 Antibody [NBP2-17036]](https://resources.bio-techne.com/images/products/Keap1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17036-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Keap1 Antibody [NBP2-17036]")
Immunocytochemistry/ Immunofluorescence: Keap1 Antibody [NBP2-17036]
Immunocytochemistry/Immunofluorescence: Keap1 Antibody [NBP2-17036] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KEAP1 protein stained by KEAP1 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Keap1 Antibody [NBP2-17036] -
Western Blot: Keap1 Antibody [NBP2-17036] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Keap1 antibody [C1C3] (NBP2-17036) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for Keap1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Keap1
Long Name
Kelch-like ECH-associated Protein 1
Alternate Names
INrf2
Gene Symbol
KEAP1
UniProt
Additional Keap1 Products
Product Documents for Keap1 Antibody
Product Specific Notices for Keap1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.