Ki67/MKI67 Antibody (SP6) - Unpurified
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1252
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Equine
Cited:
Human, Mouse, Rat, Canine
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Knockdown Validated, Western Blot
Cited:
IF/IHC, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # SP6
Format
Unpurified
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
The immunogen for this unpurified KI67/MKI67 Antibody (SP6) was made using a synthetic peptide from the C-Terminus of Human KI67/MKI67.
Epitope
C-terminus
Reactivity Notes
Rat reactivity reported in scientific literature (PMID:32731460). Ki67/MKI67 (SP6). antibody reacted with Mouse (PMID: 22020958). and Rat (PMID: 30810241). Equine and Canine reactivity reported from a verified customer review.
Localization
Nuclear
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
359 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ki67/MKI67 Antibody (SP6) - Unpurified
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252]
Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - T47D cells form functional 3D spheroids in a scaffold-free system. Sections of 3D microtissues grown for 12 days were incubated with DAPI and an antibody against Ki67 (green) to stain proliferative cells analyzed by fluorescence microscopy. Nuclei were visualized with DAPI (blue). Image collected and cropped by CiteAb from the following publication (https://doi.wiley.com/10.1002/cam4.630), licensed under a CC-BY license.Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252]
Immunohistochemistry: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - PW-12 is efficacious in an orthotopic flank tumor model of MYCN-amplified medulloblastoma. Mice were implanted with 6 x 106 SmoWT medulloblastoma cells in the hindflank. Animals were treated for 7 days with 25 mg/kg of PW-12 (n = 5) or vehicle alone (n = 5). Histology on H&E stain is consistent with medulloblastoma (H&E, 20x). Tumor cell proliferation (Ki67, 40x) was decreased, apoptosis (cleaved-caspase-3, 40x) increased, MYCN (20x) expression downregulated, and vascularity decreased in PW-12 treated tumors as confirmed by histology and cell quantification for apoptosis and cell proliferation. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/Cancer_Molecular_Targets_and_Therapeutics/10.3389/fonc.2015.00111/abstract), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252]
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (SP6) - Unpurified [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody.Applications for Ki67/MKI67 Antibody (SP6) - Unpurified
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:25-1:50
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:25-1:50
Application Notes
Use in IHC-WHMT reported in scientific literature (PMID:35104247) IHC-P: recommended incubation time of 30-60 min at RT. Ki67/MKI67 Antibody (SP6) was used for ICC/IF (PMID: 20235278) and IHC-Fr reported in scientific literature (PMID: 23300752). Use in Western blot reported in scientific literature (PMID: 31078687). Use In vivo reported in scientific literature (PMID:31398954)..
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 8 reviews rated 4.5 using NB600-1252 in the following applications:
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Format
Unpurified
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Ki67/MKI67
Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.
References
1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421
2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996
3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273
4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833
5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620
Long Name
Antigen Identified by Monoclonal Antibody Ki67
Alternate Names
Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126
Gene Symbol
MKI67
Additional Ki67/MKI67 Products
Product Documents for Ki67/MKI67 Antibody (SP6) - Unpurified
Product Specific Notices for Ki67/MKI67 Antibody (SP6) - Unpurified
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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