Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all KIDINS220 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1721 and 1771 of human Ankyrin Repeat-Rich Membrane Spanning using the numbering given in entry NP_065789.1 (GeneID 57498).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KIDINS220 Antibody - BSA Free

Western Blot: KIDINS220 Antibody [NBP1-50035]

Western Blot: KIDINS220 Antibody [NBP1-50035]

Western Blot: KIDINS220 Antibody [NBP1-50035] - Detection of Human ARMS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARMS antibod used for IP at 6 mcg/mg lysate. ARMS was also immunoprecipitated by rabbit anti-ARMS antibody which recognizes an upstream epitope. For blotting immunoprecipitated ARMS was used at 1 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for KIDINS220 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KIDINS220

A novel protein kinase D-interacting substrate of 220 kDa (Kidins220) is the first identified Protein kinase D (PKD), a serine/threonine kinase. Kidins220 is an integral membrane protein that is expressed in brain and neuroendocrine cells. Kidins220 is phosphorylated by PKD at serine 919 in vivo. Kidins220 has also been shown to function downstream of the Trks and Eph receptors.

Alternate Names

kinase D-interacting substrate, 220kDa

Gene Symbol

KIDINS220

Additional KIDINS220 Products

Product Documents for KIDINS220 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KIDINS220 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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