KIDINS220 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88995

Novus Biologicals, part of Bio-Techne
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NBP1-88995
NBP1-88995-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KIDINS220 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EPLLEIDGDIRNFEVFLSSRTPVLVARDVKVFLPCTVNLDPKLREIIADVRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KIDINS220 Antibody

Western Blot: KIDINS220 Antibody [NBP1-88995]

Western Blot: KIDINS220 Antibody [NBP1-88995]

Western Blot: KIDINS220 Antibody [NBP1-88995] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: KIDINS220 Antibody [NBP1-88995]

Immunocytochemistry/ Immunofluorescence: KIDINS220 Antibody [NBP1-88995]

Immunocytochemistry/Immunofluorescence: KIDINS220 Antibody [NBP1-88995] - Staining human cell line HEK 293 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KIDINS220 Antibody [NBP1-88995]

Immunohistochemistry-Paraffin: KIDINS220 Antibody [NBP1-88995]

Immunohistochemistry-Paraffin: KIDINS220 Antibody [NBP1-88995] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Applications for KIDINS220 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KIDINS220

KIDINS220 is a novel protein kinase D-interacting substrate of 220 kDa (Kidins220) is the first identified Protein kinase D (PKD), a serine/threonine kinase. Kidins220 is an integral membrane protein that is expressed in brain and neuroendocrine cells. Kidins220 is phosphorylated by PKD at serine 919 in vivo. Kidins220 has also been shown to function downstream of the Trks and Eph receptors.

Alternate Names

kinase D-interacting substrate, 220kDa

Gene Symbol

KIDINS220

Additional KIDINS220 Products

Product Documents for KIDINS220 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KIDINS220 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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