KIF20A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87176

Novus Biologicals, part of Bio-Techne
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NBP1-87176
NBP1-87176-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KIF20A Antibodies »

Product Summary for KIF20A Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KIF20A Antibody

Western Blot: KIF20A Antibody [NBP1-87176]

Western Blot: KIF20A Antibody [NBP1-87176]

Western Blot: KIF20A Antibody [NBP1-87176] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Western Blot: KIF20A Antibody [NBP1-87176]

Western Blot: KIF20A Antibody [NBP1-87176]

Western Blot: KIF20A Antibody [NBP1-87176] - Analysis in control (vector only transfected HEK293T lysate) and KIF20A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: KIF20A Antibody [NBP1-87176]

Immunohistochemistry-Paraffin: KIF20A Antibody [NBP1-87176]

Immunohistochemistry-Paraffin: KIF20A Antibody [NBP1-87176] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Applications for KIF20A Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KIF20A

KIF20A is a member of the kinesin-like protein family and has 1 kinesin motor domain. It interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B and may act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. KIF20A has a microtubule plus end-directed motility.

Alternate Names

FLJ21151, GG10_2, kinesin family member 20A, kinesin-like protein KIF20A, mitotic kinesin-like protein 2, MKLP2, Rab6-interacting kinesin-like protein, RAB6KIFLkinesin-like (rabkinesin6), rabkinesin-6

Entrez Gene IDs

10112 (Human)

Gene Symbol

KIF20A

UniProt

O95235 (Human)

Additional KIF20A Products

Product Documents for KIF20A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KIF20A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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