Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S112

Concentration

1 mg/ml

View all Kir2.1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1

Localization

Membrane

Specificity

Detects approx 45 kDa. No cross-reactivity against Kir2.2 or Kir2.3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Kir2.1 Antibody (S112)

Western Blot: Kir2.1 Antibody (S112) [NBP2-12900]

Western Blot: Kir2.1 Antibody (S112) [NBP2-12900]

Western Blot: Kir2.1 Antibody (S112) [NBP2-12900] - Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900]

Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900]

Immunocytochemistry/Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody (NBP2-12900) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.
Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900]

Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900]

Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
View 1 more images

Applications for Kir2.1 Antibody (S112)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Simple Western

1:100

Western Blot

1:1000
Application Notes
1 ug/mL of Kir2.1 Antibody was sufficient for detection of Kir2.1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. This Kir2.1 Antibody (S112B-14) is validated for Simple Western from a verified customer review. See Simple Western Antibody Database for Simple Western validation: tested in rat primary neonatal ventricular cardiomyocytes; antibody dilution of 1:100; separated by size
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NBP2-12900 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kir2.1

1 ug/mL was sufficient for detection of Kir2.1 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

Inward Rectifier K(+) Channel Kir2.1

Alternate Names

ATFB9, HIRK1, IRK1, KCNJ2, LQT7, SQT3

Gene Symbol

KCNJ2

Additional Kir2.1 Products

Product Documents for Kir2.1 Antibody (S112)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kir2.1 Antibody (S112)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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