KISS1 Antibody (1F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00003814-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Metastin/KiSS1 Antibodies »

Product Specifications

Immunogen

KISS1 (NP_002247, 46 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRGWGAGAGQ

Specificity

KISS1 - KiSS-1 metastasis-suppressor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for KISS1 Antibody (1F7)

Western Blot: KISS1 Antibody (1F7) [H00003814-M05]

Western Blot: KISS1 Antibody (1F7) [H00003814-M05]

Western Blot: KISS1 Antibody (1F7) [H00003814-M05] - Analysis of KISS1 expression in transfected 293T cell line by KISS1 monoclonal antibody (M05), clone 1F7. Lane 1: KISS1 transfected lysatE (14.7 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: KISS1 Antibody (1F7) [H00003814-M05]

Immunohistochemistry-Paraffin: KISS1 Antibody (1F7) [H00003814-M05]

Immunohistochemistry-Paraffin: KISS1 Antibody (1F7) [H00003814-M05] - Analysis of monoclonal antibody to KISS1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml
ELISA: KISS1 Antibody (1F7) [H00003814-M05]

ELISA: KISS1 Antibody (1F7) [H00003814-M05]

ELISA: KISS1 Antibody (1F7) [H00003814-M05] - Detection limit for recombinant GST tagged KISS1 is approximately 0.3ng/ml as a capture antibody.

Applications for KISS1 Antibody (1F7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Metastin/KiSS1

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues.

Long Name

KiSS-1 Metastasis-suppressor

Alternate Names

KiSS1, Kisspeptin-1

Entrez Gene IDs

3814 (Human)

Gene Symbol

KISS1

OMIM

603286 (Human)

UniProt

NP_002247 (Human)

Additional Metastin/KiSS1 Products

Product Documents for KISS1 Antibody (1F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KISS1 Antibody (1F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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