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KLF12 Antibody (PCRP-KLF12-1E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13848

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-13848-100ug
NBP3-13848-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-KLF12-1E3

Concentration

0.2 mg/ml

Product Summary for KLF12 Antibody (PCRP-KLF12-1E3)

Immunogen

Recombinant full-length human KLF12 protein (Uniprot: Q9Y4X4)

Reactivity Notes

Predicted to react with Mouse and Rat.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14277)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for KLF12 Antibody (PCRP-KLF12-1E3)

Immunocytochemistry/ Immunofluorescence: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848]

Immunocytochemistry/ Immunofluorescence: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848]

Immunocytochemistry/Immunofluorescence: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848] - Immunofluorescence analysis of PFA-fixed HeLa cells. KLF12 Antibody (PCRP-KLF12-1E3) followed by goat anti-mouse IgG-CF488 (green).
Flow Cytometry: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848]

Flow Cytometry: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848]

Flow Cytometry: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848] - Flow cytometric analysis of PFA-fixed HeLa cells. KLF12 antibody (PCRP-KLF12-1E3) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Protein Array: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848]

Protein Array: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848]

Protein Array: KLF12 Antibody (PCRP-KLF12-1E3) [NBP3-13848] - Analysis of Protein Array containing more than 19,000 full-length human proteins using KLF12 Antibody (PCRP-KLF12-1E3).

Applications for KLF12 Antibody (PCRP-KLF12-1E3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: KLF12

KLF12 is a gene the codes for a protein that bonds to A32 in the AP-2-alpha gene promoter in order to facilitate the transcriptional repression to the AP-2-alpha gene. KLF12 has two isoforms, with lengths of 402 and 269 amino acids, and weights of approximately 44 and 28 kDa respectively. Current studies are being done on diseases and disorders relating to this gene, including Wegener's granulomatosis, rheumatoid arthritis, gastric cancer, pancreatic cancer, pancreatitis, and schizophrenia. KLF12 has also been shown to have interactions with EHMT2, CTBP1, and SIRT2.

Long Name

Kruppel-Like Factor 12

Alternate Names

AP2REP, HSPC122

Gene Symbol

KLF12

UniProt

Additional KLF12 Products

Product Documents for KLF12 Antibody (PCRP-KLF12-1E3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLF12 Antibody (PCRP-KLF12-1E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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