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KLF2 Antibody (CL14502) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44350

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL14502

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q9Y5W3, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KLF2 Antibody (CL14502) - Azide and BSA Free

KLF2 Antibody (CL14502) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: KLF2 Antibody [NBP3-44350]

Immunocytochemistry/ Immunofluorescence: KLF2 Antibody [NBP3-44350]

Staining in U-251MG cell line with Anti-KLF2 monoclonal antibody) .
KLF2 Antibody (CL14502) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF2 Antibody [NBP3-44350]

Immunohistochemistry-Paraffin: KLF2 Antibody [NBP3-44350]

Analysis in human lymph node and cerebral cortex tissues using NBP3-44350 antibody. Corresponding KLF2 RNA-seq data are presented for the same tissues.
KLF2 Antibody (CL14502) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF2 Antibody [NBP3-44350]

Immunohistochemistry-Paraffin: KLF2 Antibody [NBP3-44350]

Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.

Applications for KLF2 Antibody (CL14502) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF2

KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Long Name

Kruppel-Like Factor 2

Alternate Names

LKLF

Gene Symbol

KLF2

Additional KLF2 Products

Product Documents for KLF2 Antibody (CL14502) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLF2 Antibody (CL14502) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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