KLF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1018

Novus Biologicals, part of Bio-Techne
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NB100-1018
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all KLF3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence LMFDPVPVKQEAMD-C corresponding to N-Terminus according to NP_057615.3.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for KLF3 Antibody

Western Blot: KLF3 Antibody [NB100-1018]

Western Blot: KLF3 Antibody [NB100-1018]

Western Blot: KLF3 Antibody [NB100-1018] - Staining of Human Heart lysate. Antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: KLF3 Antibody [NB100-1018]

Immunocytochemistry/ Immunofluorescence: KLF3 Antibody [NB100-1018]

Immunocytochemistry/Immunofluorescence: KLF3 Antibody [NB100-1018] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: KLF3 Antibody [NB100-1018]

Flow Cytometry: KLF3 Antibody [NB100-1018]

Flow Cytometry: KLF3 Antibody [NB100-1018] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for KLF3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 40 kDa band observed in human heart lysates (predicted MW of 40 kDa band according to NP_057615). Please note that the 40 kDa band is sometimes accompanied by a 80-90 kDa band of unknown identity.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLF3

The human homolog is a transcription factor involved in haematopoietic differentiation. It binds to the CACCC box.

Alternate Names

Basic krueppel-like factor, basic Kruppel-like factor, BKLFbasic kruppel like factor, CACCC-box-binding protein BKLF, Krueppel-like factor 3, Kruppel-like factor 3 (basic), MGC48279, TEF-2

Entrez Gene IDs

51274 (Human)

Gene Symbol

KLF3

UniProt

NP_057615. (Human)

Additional KLF3 Products

Product Documents for KLF3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KLF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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