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KLF4 Antibody (3A1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26180

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26180-100ul
NBP3-26180-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3A1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for KLF4 Antibody (3A1)

Immunogen

A synthesized peptide derived from Human KLF4 [UniProt O43474]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF4 Antibody (3A1)

KLF4 Antibody (3A1)

Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] -

Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] - Immunoprecipitating KLF4 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26180 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26180 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
KLF4 Antibody (3A1)

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] -

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
KLF4 Antibody (3A1)

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] -

Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for KLF4 Antibody (3A1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Gene Symbol

KLF4

Additional KLF4 Products

Product Documents for KLF4 Antibody (3A1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLF4 Antibody (3A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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