KLF4 Antibody (4E6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21047

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E6

Format

BSA Free

Concentration

1.0 mg/ml

View all KLF4 Antibodies »

Product Specifications

Immunogen

Recombinant human KLF4 (1-170aa) purified from E. coli (NP_004226.3).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLF4 Antibody (4E6) - BSA Free

Western Blot: KLF4 Antibody (4E6)BSA Free [NBP1-21047]

Western Blot: KLF4 Antibody (4E6)BSA Free [NBP1-21047]

Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Flow Cytometry: KLF4 Antibody (4E6) - BSA Free [NBP1-21047]

Flow Cytometry: KLF4 Antibody (4E6) - BSA Free [NBP1-21047]

Flow Cytometry: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in A549 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Western Blot: KLF4 Antibody (4E6)BSA Free [NBP1-21047]

Western Blot: KLF4 Antibody (4E6)BSA Free [NBP1-21047]

Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - The recombinant protein (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human KLF4 protein

Applications for KLF4 Antibody (4E6) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1x10^6cells

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF4

KLF4, which is also known as the gut-enriched Kruppel-like factor (GKLF), is a zinc finger-containing transcription factor that belongs to the Kruppel-like family of transcription factors. KLF4 plays important roles during the proliferation and differentiation of epithelial cells. Especially, it is found predominantly in gut and has been shown to be expressed during growth arrest. KLF4 is also involved in cell cycle control since expression of KLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Entrez Gene IDs

9314 (Human)

Gene Symbol

KLF4

UniProt

O43474 (Human)

Additional KLF4 Products

Product Documents for KLF4 Antibody (4E6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KLF4 Antibody (4E6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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