KLF4 Antibody (7B7U1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15469
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7B7U1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for KLF4 Antibody (7B7U1)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KLF4 (O43474). PAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KLF4 Antibody (7B7U1)
Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469]
Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of Mouse lung, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469]
Immunocytochemistry/Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469] - Immunofluorescence analysis of U-2 OS cells using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469]
Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Mouse spinal cord using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for KLF4 Antibody (7B7U1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: KLF4
Long Name
Kruppel-Like Factor 4
Alternate Names
EZF
Gene Symbol
KLF4
Additional KLF4 Products
Product Documents for KLF4 Antibody (7B7U1)
Product Specific Notices for KLF4 Antibody (7B7U1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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