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KLF4 Antibody (CL5782) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44207

Novus Biologicals, part of Bio-Techne
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NBP3-44207

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL5782

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O43474, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for KLF4 Antibody (CL5782) - Azide and BSA Free

KLF4 Antibody (CL5782) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44207]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44207]

Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).
KLF4 Antibody (CL5782) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44207]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44207]

Staining of human prostate shows nuclear immunoreactivity in glandular cells.
KLF4 Antibody (CL5782) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44207]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44207]

Staining of human placenta shows nuclear positivity in the trophoblast.

Applications for KLF4 Antibody (CL5782) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Gene Symbol

KLF4

Additional KLF4 Products

Product Documents for KLF4 Antibody (CL5782) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KLF4 Antibody (CL5782) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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