KLF4 Antibody

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of KLF4 [red] on the cells within the tumor nests (TNs) [thin arrows] and those within the peritumoral stroma [arrowheads]. OCT4 [ green] was expressed on the KLF4+ [green, arrowheads] cells within the peritumoral stroma, and also cells within the peritumoral stroma that expressed KLF4 [ red, thick arrows] not expressing OCT4. c-Myc was expressed by cells within the TNs [green, arrows] and those within the peritumoral stroma [arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed KLF4 [red]. Cell nuclei were counterstained with DAPI [blue]. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license.

Flow Cytometry: KLF4 Antibody [NBP2-24749]

Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an anti-rabbit IgG PE conjugated secondary .

Western Blot: KLF4 Antibody [NBP2-24749]

Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the (1) absence and (2) presence of immunizing peptide, (3) mouse NIH 3T3, and (4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). Goat anti-rabbit IgG HRP secondary and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] -

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative 3,3-diaminobenzidine immunohistochemical stained images of WHO grade I meningioma for OCT4 (A, brown), NANOG (B, purple), SOX2 (C, brown), KLF4 (D, purple) & c-MYC (E, brown) expressed on the endothelial (red arrows) & pericyte (black arrows) layers. Cell nuclei were counterstained with hematoxylin (A-E, blue). Original magnification: 400X. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: KLF4 Antibody [NBP2-24749] -

Western Blot: KLF4 Antibody [NBP2-24749] - Representative Western blot images of total protein extracted from four head & neck metastatic malignant melanoma-derived primary cell lines probed for induced pluripotent stem cell markers. Blots were probed for OCT4 (A), NANOG (B), SOX2 (C), KLF4 (D), & c-MYC (E) & detected with HRP conjugated goat anti-rabbit antibody. alpha-Tubulin was used as the loading control (F) & detected using HRP conjugated mouse IgGĸ binding protein. Arrows indicate the presence of the proteins with their expected band sizes. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32019273), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: KLF4 Antibody [NBP2-24749] -

Immunohistochemistry: KLF4 Antibody [NBP2-24749] - Representative immonoflourescence immunohistochemical stained images of WHO grade I meningioma demonstrating the expression of SOX2 (A, red), NANOG (B, red), KLF4 (C, red), OCT4 (D, green) & c-MYC (E, green) on both the SMA+ (A–C, red, red arrows) pericyte layer, & the ERG+ endothelial layer (D,E, red, white arrows). Cell nuclei were counterstained with 4′, 6′-diamino-2-phenylindole (A–F), blue. Original magnification: 600X. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] -

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative colorimetric in situ hybridization stained images of WHO grade I meningioma demonstrating mRNA transcript expression for OCT4 (A, brown), NANOG (B, brown), SOX2 (C, brown), KLF4 (D, brown) & c-MYC (E, brown). Nuclei were counterstained with hematoxylin (A-E, blue). Orignal magnification: 1,000X. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] -

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of SOX2 [(A,D), red], NANOG [(B,E), red], KLF4 [(C,F), red], & c-Myc [(D–F), green] on the cells within the tumor nests (TNs) [(A–F), thin arrows] & those within the peritumoral stroma [(A–F), arrowheads]. OCT4 [(A–C), green] was expressed on the SOX2+ [(A), green, arrowheads], NANOG+ [(B), green, arrowheads], & KLF4+ [(C), green, arrowheads] cells within the peritumoral stroma. There were also cells within the peritumoral stroma that expressed SOX2 [(A), red, thick arrows], NANOG [(B), red, thick arrows] & KLF4 [(C), red, thick arrows] that did not express OCT4. c-Myc was expressed by cells within the TNs [(D–F), green, arrows] & those within the peritumoral stroma [(D–F), arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed SOX2 [(D), red], NANOG [(E), red], & KLF4 [(F), red]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] -

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of SOX2 [(A,D), red], NANOG [(B,E), red], KLF4 [(C,F), red], & c-Myc [(D–F), green] on the cells within the tumor nests (TNs) [(A–F), thin arrows] & those within the peritumoral stroma [(A–F), arrowheads]. OCT4 [(A–C), green] was expressed on the SOX2+ [(A), green, arrowheads], NANOG+ [(B), green, arrowheads], & KLF4+ [(C), green, arrowheads] cells within the peritumoral stroma. There were also cells within the peritumoral stroma that expressed SOX2 [(A), red, thick arrows], NANOG [(B), red, thick arrows] & KLF4 [(C), red, thick arrows] that did not express OCT4. c-Myc was expressed by cells within the TNs [(D–F), green, arrows] & those within the peritumoral stroma [(D–F), arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed SOX2 [(D), red], NANOG [(E), red], & KLF4 [(F), red]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.