KLHL2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17080

Novus Biologicals, part of Bio-Techne
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NBP2-17080
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KLHL2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLHL2. The exact sequence is proprietary.

Localization

Cytoplasm; cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLHL2 Antibody

Western Blot: KLHL2 Antibody [NBP2-17080]

Western Blot: KLHL2 Antibody [NBP2-17080]

Western Blot: KLHL2 Antibody [NBP2-17080] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody [NBP2-17080]

Immunocytochemistry/ Immunofluorescence: KLHL2 Antibody [NBP2-17080]

Immunocytochemistry/Immunofluorescence: KLHL2 Antibody [NBP2-17080] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using KLHL2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: KLHL2 Antibody [NBP2-17080]

Immunohistochemistry-Paraffin: KLHL2 Antibody [NBP2-17080]

Immunohistochemistry-Paraffin: KLHL2 Antibody [NBP2-17080] - Human colon carcinoma, using KLHL2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for KLHL2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KLHL2

KLHL2, also known as Kelch-like protein 2, has a 593 short amino acid that is 66 kDa and a long 597 amino acid that is 67 kDa, predominantly expressed in brain, and may participate in organizing the actin cytoskeleton of the brain cells. This protein is currently being studied for research on the following diseases and disorders: contagious pustular dermatitis, lumpy skin disease, monkeypox, smallpox, cowpox, vaccinia, skin disease, dermatitis, pharyngitis, breast cancer, and neuronitis. The protein has been shown to interact with KLHL12 in intracellular protein transport pathways.

Alternate Names

ABP-KELCH, Actin-binding protein Mayven, kelch, kelch (Drosophila)-like 2 (Mayven), kelch-like 2, Mayven (Drosophila), kelch-like protein 2, MAV, MAYVEN

Gene Symbol

KLHL2

UniProt

O95198 (Human)

Additional KLHL2 Products

Product Documents for KLHL2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KLHL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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