KMT2A/MLL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-248
![Western Blot: KMT2A/MLL Antibody [NB600-248]](https://resources.bio-techne.com/images/products/KMT2A-MLL-Antibody-Western-Blot-NB600-248-img0008.jpg "Western Blot: KMT2A/MLL Antibody [NB600-248]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chemotaxis, Immunohistochemistry-Paraffin, Immunoprecipitation, RNA immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 720 and 780 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31786140).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KMT2A/MLL Antibody
Western Blot: KMT2A/MLL Antibody [NB600-248]
Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: KMT2A/MLL Antibody [NB600-248]](https://resources.bio-techne.com/images/products/KMT2A-MLL-Antibody-Immunoprecipitation-NB600-248-img0009.jpg "Immunoprecipitation: KMT2A/MLL Antibody [NB600-248]")
Immunoprecipitation: KMT2A/MLL Antibody [NB600-248]
Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-249. For blotting immunoprecipitated MLL1, NB600-248 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for KMT2A/MLL Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:250
Immunoprecipitation
2 - 10 ug/mg of lysate
Western Blot
1:500-1:5000
Application Notes
Use in IHC-P reported in scientific literature (PMID: 27396339).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NB600-248 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: KMT2A/MLL
Alternate Names
ALL1, ALL-1MLL/GAS7, CXXC7TET1-MLL, CXXC-type zinc finger protein 7, EC 2.1.1.43, HRXFLJ11783, HTRX, HTRX1MLL-AF4 der(11) fusion protein, KMT2ACDK6/MLL fusion protein, Lysine N-methyltransferase 2A, MLL/GAS7 fusion protein, MLL/GMPS fusion protein, MLL1, MLL1A, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), Trithorax-like protein, TRX1histone-lysine N-methyltransferase MLL, Zinc finger protein HRX
Gene Symbol
KMT2A
UniProt
Additional KMT2A/MLL Products
Product Documents for KMT2A/MLL Antibody
Product Specific Notices for KMT2A/MLL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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