Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F8

Format

BSA Free

Concentration

1 mg/ml

View all KRAS Antibodies »

Product Specifications

Immunogen

Recombinant human KRAS (1-186aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for KRAS Antibody (2F8) - BSA Free

Western Blot: KRAS Antibody (2F8)BSA Free [NBP2-59413]

Western Blot: KRAS Antibody (2F8)BSA Free [NBP2-59413]

Western Blot: KRAS Antibody (2F8) [NBP2-59413] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate
Immunocytochemistry/ Immunofluorescence: KRAS Antibody (2F8) - BSA Free [NBP2-59413]

Immunocytochemistry/ Immunofluorescence: KRAS Antibody (2F8) - BSA Free [NBP2-59413]

Immunocytochemistry/Immunofluorescence: KRAS Antibody (2F8) [NBP2-59413] - Analysis of RAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: KRAS Antibody (2F8)BSA Free [NBP2-59413]

Western Blot: KRAS Antibody (2F8)BSA Free [NBP2-59413]

Western Blot: KRAS Antibody (2F8) [NBP2-59413] - Lane 1: KRAS recombinant protein, Lane 2: HRAS recombinant protein, Lane 3: NRAS recombinant protein

Applications for KRAS Antibody (2F8) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KRAS

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Alternate Names

cellular c-Ki-ras2 proto-oncogene, c-Ki-ras, c-K-ras, GTPase KRas, Ki-Ras, Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog, K-Ras 2, K-ras p21 protein, KRAS1, K-RAS2A, K-RAS2B, KRAS2NS3, K-RAS4A, K-RAS4B, NS, oncogene KRAS2, PR310 c-K-ras oncogene, RASK2c-Kirsten-ras protein, transforming protein p21, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Gene Symbol

KRAS

Additional KRAS Products

Product Documents for KRAS Antibody (2F8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KRAS Antibody (2F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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